ABC112

Anti-phospho-ULK1/ATG1 (Ser758) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• técnica(s): inhibition assay: suitable (peptide); western blot: suitable
• nº de adesão NCBI: NP_003556
• nº de adesão UniProt: O75385
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer758)
• Informações sobre genes: human ... ULK1(8408)

Descrição

Descrição geral

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Especificidade

This antibody recognizes the ULK1/ATG1 phosphorylated at Ser758.

Imunogênio

Epitope: Phosphorylated Ser758

KLH-conjugated linear peptide corresponding to human ULK1/ATG1 phosphorylated at Ser758.

Aplicação

Detect phospho-ULK1/ATG1 (Ser758) using this Anti-phospho-ULK1/ATG1 (Ser758) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.

Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-ULK1/ATG1 (Ser758) in serum-starved A431 cell lysates.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Qualidade

Evaluated by Western Blot in A431 and serum starved A431 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected ULK1/ATG1 on 10 µg of A431 and serum starved A431 cell lysates..

Descrição-alvo

~120 kDa observed

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
A431 and serum starved A431 cell lysates.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable