AB5895
Anti-P2X3 Receptor Antibody, pain
serum, Chemicon®
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
serum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
reatividade de espécies
rat, human, primate
fabricante/nome comercial
Chemicon®
técnica(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... P2RX3(5024)
Especificidade
P2X3
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.
Imunogênio
Synthetic peptide corresponding to the C-terminus of rat P2X3.
Aplicação
Detect P2X3 Receptor using this Anti-P2X3 Receptor Antibody, pain validated for use in IC, IH & WB.
Immunoblotting: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000
Immunocytochemistry: 1:500-1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5895
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRY
P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Immunohistochemistry: 1:500-1:1,000
Immunocytochemistry: 1:500-1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5895
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRY
P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuroinflammation & Pain
Neurotransmitters & Receptors
Neuroinflammation & Pain
forma física
Rabbit serum. Liquid. Contains 0.05% sodium azide.
Armazenamento e estabilidade
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
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P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization.
Deiteren, A; van der Linden, L; de Wit, A; Ceuleers, H; Buckinx, R; Timmermans et al.
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Da Fei et al.
PloS one, 8(12), e83460-e83460 (2014-01-05)
Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) are two neurotrophins that play distinct roles in geniculate (taste) neuron survival, target innervation, and taste bud formation. These two neurotrophins both activate the tropomyosin-related kinase B (TrkB) receptor and the pan-neurotrophin receptor
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Advances in experimental medicine and biology, 758, 157-166 (2012-10-20)
We recently developed an ex vivo lung slice model that allows for confocal live cell imaging (LCI) of neuroepithelial bodies (NEBs) in postnatal mouse lungs (postnatal days 1-21 and adult). NEBs are morphologically well-characterized, extensively innervated groups of neuroendocrine cells
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