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AB5519

Sigma-Aldrich

Anti-Prodynorphin Antibody

Chemicon®, from guinea pig

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

guinea pig

Nível de qualidade

100

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

rat

fabricante/nome comercial

Chemicon®

técnica(s)

immunohistochemistry: suitable

nº de adesão NCBI

NM_024411.2

nº de adesão UniProt

P01213

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PDYN(5173)

Especificidade

ProDynorphin, rat.

Imunogênio

Synthetic peptide from rat ProDynorphin.

Aplicação

Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.
Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5519

IMMUNOHISTOCHEMISTRY

Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

forma física

Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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