AB3098

Anti-Orexin-A Antibody

Chemicon^®, from rabbit

• Sinônimo(s): Anti-NRCLP1, Anti-OX, Anti-PPOX
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: feline, rat, human, mouse
• fabricante/nome comercial: Chemicon^®
• técnica(s): ELISA: suitable; western blot: suitable
• nº de adesão NCBI: NM_001524.1
• nº de adesão UniProt: O43612
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PPOX(5498)

Descrição

Especificidade

Mouse Orexin-A. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Imunogênio

An 18 amino acid peptide mapping near the amino terminus of mouse Orexin-A. The immunogen sequence is identical in human, rat, porcine, ovine, bovine and canine and has 81% homology with chicken.

Aplicação

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

This Anti-Orexin-A Antibody is validated for use in ELISA, WB for the detection of Orexin-A.

Western blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 1:10,000 - 100,000 using 5-100 ng of control peptide per well. NOTE: Orexin A is a 3.5kDa protein. Adjust your SDS-PAGE gel and lane size markers accordingly.

Immunohistochemistry: Frozen 4% PFA tissue with ABC detection {Wagner et al 2000}.

Optimal working dilutions must be determined by end user.

forma física

Liquid in PBS, pH 7.4 with 0.1% BSA and 0.1% sodium azide.

Armazenamento e estabilidade

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable