AB3080

Anti-Green Fluorescent Protein Antibody

Chemicon^®, from rabbit

• Sinônimo(s): GFP, eGFP
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: vertebrates
• fabricante/nome comercial: Chemicon^®
• técnica(s): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão UniProt: P42212
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Especificidade

AB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.

Imunogênio

Highly purified native GFP from Aequorea victoria.

Aplicação

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

This Anti-Green Fluorescent Protein Antibody is validated for use in ELISA, IC, IH, WB for the detection of Green Fluorescent Protein.

Western Blot:
A previous lot of this antibody was used in western blot.

ELISA:
A 1:2000-1:10,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay.

Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore’s highly-purified secondary antibodies in indirect detection systems.

Immunocytochemistry:

Optimal working dilutions must be determined by end user.

Qualidade

Routinely evaluated by immunocytochemistry on RENCX cells.

Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot of anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.

Descrição-alvo

GFP is 27 kDa Dependent upon the molecular weight of the fusion protein being detected.

forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in 10 mM Tris buffer, pH 7.2 with 0.01% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
GFP fusion protein expressed in cells

RENCX cells

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable