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AB1765P

Sigma-Aldrich

Anti-Dopamine D1A Receptor Antibody, CT, cytoplasmic

Chemicon®, from rabbit

Sinônimo(s):

Anti-dadr

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

rat, pig

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... DRD1(1812)

Especificidade

Dopamine Receptor D1A. An antibody made to the D1R peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes by Western blot. The 75 kDa band was not visible in striatum (Huang et al. 1992). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band. By immunohistochemistry D1R has been localized in various regions of the rat brain (Huang et al. 1992).

May recognize porcine or bovine (100 % similarity) or human (78% similarity).

Imunogênio

A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et al. 1990; Zhou et al. 1990; Zhou et al. 1992; O′Dowd et al. 1990). This peptide is predicted to be cytoplasmic.
Epitope: C-terminus, cytoplasmic

Aplicação

Anti-Dopamine D1A Receptor Antibody, C-terminus, cytoplasmic is an antibody against Dopamine D1A Receptor for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang et al. 1992; O′Connel et al. 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang et al. 1992; O′Connel et al. 1995) that is present in relatively small amounts.

Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL D1A peptide (Cat. Number AG259)/well)

Optimal working dilutions must be determined by end user.

forma física

Affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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