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Documentos Principais

AB143

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

CHEMICON®, rabbit polyclonal

Sinônimo(s):

Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Nome do produto

Anti-Choline Acetyltransferase (ChAT) Antibody, serum, Chemicon®

fonte biológica

rabbit

Nível de qualidade

100

forma do anticorpo

serum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

mouse, human, feline, bat, rat, monkey

fabricante/nome comercial

Chemicon®

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

nº de adesão NCBI

NP_065574

nº de adesão UniProt

P28329

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

cat ... Chat(751512)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

Descrição geral

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Especificidade

Cholinergic neurons in brain and central nervous systems.
Reactivity with other species has not been confirmed with IHC.

Aplicação

Anti-Choline Acetyltransferase (ChAT) Antibody is an antibody against Choline Acetyltransferase (ChAT) for use in IH, IH(P), IP & WB with more than 70 product citations.
Immunoprecipitation:
1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.

Optimal working dilutions and protocls must be determined by end user.

Qualidade

Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis:
ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).

forma física

Rabbit polyclonal antisera, liquid, no preservatives.

Nota de análise

Control
Cerebral cortex tissue.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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K Sasaki et al.
Neuroscience, 92(4), 1287-1294 (1999-07-30)
We examined the effects of repeated subcutaneous injections of an acidic fibroblast growth factor fragment analog, [Ala16] acidic fibroblast growth factor (1-29), on learning and memory and on the choline acetyltransferase immunoreactivity of forebrain neurons in senescence-accelerated mice. One group
B M Kramer et al.
Neuroscience, 94(4), 1163-1172 (2000-01-07)
The role of the p75 nerve growth factor receptor in the retrograde transport of neurotrophins in the adult CNS was investigated by comparing the transport of 125I-labeled neurotrophins by normal and p75 nerve growth factor receptor-deficient cholinergic septohippocampal neurons. In
U Greferath et al.
Neuroscience, 100(2), 363-373 (2000-09-29)
We investigated age-related changes in the number and size of neurons positive for the p75 neurotrophin receptor in the cholinergic basal forebrain of female Dark Agouti rats. Since the integrity of these neurons is known to be closely associated with
E Jolkkonen et al.
Neuroscience, 111(1), 133-149 (2002-04-17)
The amygdaloid complex has a key role in the modulation of behavioral responses in life-threatening situations, including the direction of attentional responses to sensory stimuli. The pathways from the amygdala to the basal forebrain cholinergic system, which projects to the
C E E M van der Zee et al.
Neuroscience, 110(4), 641-651 (2002-04-06)
The p75 low-affinity neurotrophin receptor (p75(LNTR)) appears to have various functions that include enhancing nerve growth factor (NGF)-mediated survival by increasing TrkA (high-affinity NGF receptor) efficiency, and mediating apoptosis by acting as a ligand-regulated pro-apoptotic receptor. Here, we investigated the
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