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BLR(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

Sinônimo(s):

Competent cells for cloning

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About This Item

Código UNSPSC:
41106202
NACRES:
NA.81

Nome do produto

BLR(DE3) Competent Cells - Novagen, BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

fonte biológica

Escherichia coli

Nível de qualidade

100

fabricante/nome comercial

Novagen®

condição de armazenamento

OK to freeze
avoid repeated freeze/thaw cycles

modo de crescimento

adherent or suspension

morfologia

rod shaped

técnica(s)

microbiological culture: suitable

transformação celular

transformation efficiency: >2×106 cfu/μg

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Categorias relacionadas

Descrição geral

BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

Componentes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Outras notas

1. A. Roca (U. of Wisconsin), personal
communication.

2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

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