614852

Topoisomerase IIα Inhibitor, TSC24

The Topoisomerase IIα Inhibitor, TSC24 controls the biological activity of Topoisomerase. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Sinônimo(s): Topoisomerase IIα Inhibitor, TSC24, (E)-N,N-dimethyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamide
• Fórmula empírica (Notação de Hill): C₁₃H₁₄N₄S
• Peso molecular: 258.34
• Código UNSPSC: 12352200
• NACRES: NA.54

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• forma: powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: yellow
• solubilidade: DMSO: 15 mg/mL
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A cell-permeable, TSC (thiosemicarbazone) derivative that acts as a selective and ATP competitive Topoisomerase IIα inhibitor and Fe chelator. Independent of its Fe chelating ability, this compound is shown to block Topo II&alpha-catalyzed ATP hydrolysis by competing with ATP for binding to the ATPase domain of Topo IIα (K_d = 18 µM). It also displays M phase cell-cycle arrest at 1 µM in nocodazole-treated HT-29 cells. This compound demonstrates anti-proliferative activity, without distinct or obvious selectivity, towards HeLa, HL-60, SGC7901, and HT-29 cultures (IC₅₀ = 90 nM, 50 nM, 30 nM, and 20 nM, respectively), and a broader panel of tumor cell lines with IC₅₀ values in the nanomolar range.

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Outras notas

Huang, H., et al. 2010. J. Med. Chem.53, 3048.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable