529659

DDD00033325

≥95% (HPLC), syrup, single hESC survival promoter, Calbiochem^®

• Sinônimo(s): Pro-survival Compound, DDD00033325, DDD00033325
• Fórmula empírica (Notação de Hill): C₁₇H₁₉N₅
• Número CAS: 1259034-82-6
• Peso molecular: 293.37
• Código UNSPSC: 51111800
• NACRES: NA.77

Propriedades

• Nome do produto: Pro-survival Compound, DDD00033325, A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.
• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• Formulário: syrup
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: yellow
• solubilidade: DMSO: 100 mg/mL
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: C12=CC=NN1C=CC(N[C@@H]3CCN(CC4=CC=CC=C4)C3)=N2
• InChI: 1S/C17H19N5/c1-2-4-14(5-3-1)12-21-10-7-15(13-21)19-16-8-11-22-17(20-16)6-9-18-22/h1-6,8-9,11,15H,7,10,12-13H2,(H,19,20)/t15-/m1/s1
• chave InChI: ZJVYTXWKNDTQNC-OAHLLOKOSA-N

Descrição

Descrição geral

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

Embalagem

Packaged under inert gas

Advertência

Toxicity: Regulatory Review (Z)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Outras notas

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable