524627
Phosphatase Inhibitor Cocktail III
liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases
Sinônimo(s):
Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.77
Produtos recomendados
product name
Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
forma
liquid
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Descrição geral
Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.
Aplicação
Phosphatase Inhibitor Cocktail Set III has been used:
- as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
- to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
- as a component in cell lysis buffer to extract total proteins for western blotting
Ações bioquímicas/fisiológicas
Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no
Características e benefícios
- Ready-to-use formulations for convenient use
- High-quality products for consistent and reproducible performance,
- Flexible options with a range of cocktails targeting different categories of phosphatases
Componentes
Each vial contains 1 ml of aqueous solution with the following components:
- 50 mM Sodium Fluoride
- 10 mM ß-Glycerophosphate (Cat. No. 35675)
- 10 mM Sodium Pyrophosphate Decahydrate
- 1 mM Sodium Orthovanadate
Advertência
Toxicity: Toxic (F)
forma física
In H₂O.
Reconstituição
For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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