499050

Osteocalcin, Bovine Bone

• Sinônimo(s): Osteocalcin, Bovine Bone, Bone-Gla-Protein, BGP
• Fórmula empírica (Notação de Hill): C₂₆₃H₃₇₂N₆₆O₈₂S₂
• Peso molecular: 5834.29
• Código UNSPSC: 12352202
• NACRES: NA.42

Propriedades

• Ensaio: ≥98% (SDS-PAGE)
• Nível de qualidade: 100
• forma: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 4.0 - 4.5
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. A good marker of bone turnover. May play a role in bone mineralization, bone resorption, and bone formation.

Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca²⁺ (K_d = 6 µM). Also binds hydroxylapatite. Possible role in bone mineralization, bone resorption, and bone formation. Note: Gla corresponds to γ-carboxyglutamic acid.

Advertência

Toxicity: Standard Handling (A)

Sequência

H-Tyr-Leu-Asp-His-Trp-Leu-Gly-Ala-Pro-Ala-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Lys-Arg-Gla-Val-Cys²³-Gla-Leu-Asn-Pro-Asp-Cys²⁹-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val-OH (disulfide bond: 23 → 29)

forma física

In 75 mM NaCl, 10 mM sodium phosphate buffer, pH 7.4.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Outras notas

Carter, S.D., et al. 1996. J. Anim. Sci. 74, 2719.
Watson, K.E., et al. 1994. J. Clin. Invest.93, 2106.
Tracy, R.P., et al. 1990. J. Bone Min. Res.5, 451.
Gendreau, M.A., et al. 1989. J. Biol. Chem.264, 6972.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable