480026

NBD-Binding Peptide II, Cell Permeable, CTP-NBD

A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFα-stimulated NF-κB activity (IC₅₀~ 50 µM in HCT116 cells).

• Sinônimo(s): NBD-Binding Peptide II, Cell Permeable, CTP-NBD, NEMO-Binding Domain Binding Peptide II, Cell-Permeable, CTP-NBD
• Fórmula empírica (Notação de Hill): C₁₂₃H₁₉₇N₄₇O₃₂
• Peso molecular: 2846.18
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• Formulário: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic); protect from light
• cor: white
• solubilidade: water: 50 mg/mL
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFα-stimulated NF-κB activity (IC₅₀ ~ 50 µM in HCT116 cells). Acts by interfering with the formation of active IKK complex and thereby prevents IκBα phosphorylation and its subsequent proteasomal degradation. Also blocks LPS-mediated increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activities. Colon-targeted capsules containing CTP-NBD are shown to reduce 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rodent models (4.5 mg/kg/,p.o.) in vivo and diminish activities of myeloperoxidase and several inflammatory mediators. Antennapedia fused NBD cell-permeable peptide is also available (>Cat. No. 480025).

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
NEMO-binding peptide

Reversible: yes

Embalagem

Packaged under inert gas

Yes

Advertência

Toxicity: Standard Handling (A)

Sequência

Ac-YGRRARRRARRTALDWSWLQTE-NH₂

Outras notas

Hong, S., et al. 2012. Mol. Pharm.9, 1310.
Kim, D., et al. 2006. Exp. Cell Res.312, 1277.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable