374817

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Sinônimo(s): Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, KLH
• Número CAS: 9013-72-3
• Número MDL: MFCD00131280
• Código UNSPSC: 12352202
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• Ensaio: >90% (size exclusion chromatography)
• forma: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: clear blue-gray
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.

Nota de preparo

Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Outras notas

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2