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33-017

Sigma-Aldrich

PI 3-Kinase HTRF® Assay; 1920 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.

Sinônimo(s):

Kinase activity assay

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Nível de qualidade

100

reatividade de espécies

mouse, human

fabricante/nome comercial

HTRF®
Upstate®

técnica(s)

activity assay: suitable (kinase)

nº de adesão NCBI

NM_006218.2

nº de adesão UniProt

P42336

método de detecção

fluorometric (HTRF)

Condições de expedição

dry ice

Informações sobre genes

human ... PIK3CA(5290)

Descrição geral

The PI3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for assaying all Class I PI3-Kinases in a homogeneous 384-well format.

Aplicação

PI 3-Kinase HTRF® Assay; 1920 wells have been used to measure the activity of phosphoinositide-3-kinases (PI3Ks). It has also been used in a biochemical assay to measure the potency and selectivity of LL-00071210 (a selective PI3Kδ inhibitor).

Ações bioquímicas/fisiológicas

The PI 3-Kinases catalyze the phosphorylation of the three positions of phosphatidylinositols. The Class I enzymes convert phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg2+.

Características e benefícios

  • Large Stokes shift and time-resolved elements of technology help to reduce compound interference, minimizing concern of false negatives
  • Picomole sensitivity of the HTRF assay platform enables the integrity of enzyme kinetics to be retained, ensuring the most physiologically relevant assay parameters.
  • Compatible with all Class I phosphoinositide 3-kinase (PI3K) isoforms: enables relative data comparisons and eliminates the need for multiple assay types.
  • Reliable, proven assay—based on the technology used in the Millipore, Upstate® KinaseProfiler Service.
  • Z` factor routinely exceeds 0.8, ensuring consistent, reliable data.

Embalagem

Kit capacity: 1920 wells

Componentes

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

Informações legais

HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H319

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Certificados de análise (COA)

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Robert H Sinnamon et al.
Protein expression and purification, 73(2), 167-176 (2010-05-12)
Phosphoinositide 3-kinases have been targeted for therapeutic research because they are key components of a cell signaling cascade controlling proliferation, growth, and survival. Direct activation of the PI3Kalpha pathway contributes to the development and progression of solid tumors in breast

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