324708

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

• Sinônimo(s): Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
• Número CAS: 9001-92-7
• Número da licença da enzima: 3.4.24.33 (BRENDA, IUBMB)
• Número MDL: MFCD00004464
• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• grau: Proteomics Grade
• Nível de qualidade: 100
• Ensaio: ≥90% (SDS-PAGE)
• Formulário: lyophilized solid
• atividade específica: ≥20,000 units/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white
• solubilidade: water: soluble
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Advertência

Toxicity: Harmful (C)

Definição da unidade

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

forma física

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Outras notas

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1