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Merck
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Documentos Principais

217570

Sigma-Aldrich

Anti-CD3 Mouse mAb (UCHT1)

liquid, >95% (immunoglobulin, SDS-PAGE), clone UCHT1

Sinônimo(s):

Anti-CD3 Antibody, CD3 Detection Antibody, Mouse Anti-CD3

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

mouse

Nível de qualidade

100

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

UCHT1, monoclonal

Ensaio

>95% (immunoglobulin, SDS-PAGE)

Formulário

liquid

contém

0.5 mg/mL gentamycin sulfate as preservative

reatividade de espécies

human

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze

Isotipo

IgG1

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.

Imunogênio

human thymocytes/Sezary T cells

Aplicação

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see application references)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

Outras notas

Thibault, G. and Bardos, P. 1995. J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol.11, 329.
This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Javier Robles-Valero et al.
The EMBO journal, 40(22), e108125-e108125 (2021-10-08)
Mutations in VAV1, a gene that encodes a multifunctional protein important for lymphocytes, are found at different frequencies in peripheral T-cell lymphoma (PTCL), non-small cell lung cancer, and other tumors. However, their pathobiological significance remains unsettled. After cataloguing 51 cancer-associated
Sonia Rodríguez-Fdez et al.
Cells, 9(3) (2020-03-08)
Vav1 works both as a catalytic Rho GTPase activator and an adaptor molecule. These functions, which are critical for T cell development and antigenic responses, are tyrosine phosphorylation-dependent. However, it is not known whether other posttranslational modifications can contribute to
Sonia Rodríguez-Fdez et al.
Cells, 8(12) (2020-01-01)
Phosphatidylinositol-5 phosphate (PI5P) and other mono-phosphoinositides (mono-PIs) play second messenger roles in both physiological and pathological conditions. Despite this, their intracellular targets and mechanisms of action remain poorly characterized. Here, we show that Vav1, a protein that exhibits both Rac1

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