208731

Calpain Substrate II, Fluorogenic

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

• Sinônimo(s): Calpain Substrate II, Fluorogenic, Suc-LY-AMC
• Fórmula empírica (Notação de Hill): C₂₉H₃₁N₃O₇
• Número CAS: 94367-20-1
• Peso molecular: 533.57
• Número MDL: MFCD00057900
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (TLC)
• Formulário: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated; protect from light
• cor: off-white
• solubilidade: DMSO: soluble
• fluorescência: λ_ex ~380 nm; λ_em ~460 nm
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C
• InChI: 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
• chave InChI: VSWNAJAOJZXWBM-FTJGSZSVSA-N

Descrição

Descrição geral

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

Advertência

Toxicity: Standard Handling (A)

Sequência

Suc-Leu-Tyr-AMC

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Outras notas

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable