208773

Calpain Substrate IV, Cell-Permeable, Fluorogenic

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

• Sinônimo(s): Calpain Substrate IV, Cell-Permeable, Fluorogenic
• Fórmula empírica (Notação de Hill): C₁₂₇H₂₀₆N₅₀O₂₇S
• Peso molecular: 2897.38
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• forma: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic); protect from light
• solubilidade: DMSO: 5 mg/mL
• fluorescência: λ_ex 480 nm; λ_em 320 nm
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (k_cat = 0.2 s^-1, k_cat/K_M = 5000 M^-1 s^-1) when compared to the parental peptide (k_cat = 0.17 s^-1, k_cat/K_M = 680 M^-1 s^-1). Its suitability in detecting cellular Ca²⁺-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca²⁺ addition.

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Embalagem

Packaged under inert gas

Advertência

Toxicity: Standard Handling (A)

Sequência

(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂

forma física

Supplied as a trifluoroacetate salt

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Outras notas

Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable