16-318

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

clone SKB1, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: SKB1, monoclonal
• reatividade de espécies: mouse, rat, human
• técnica(s): immunoprecipitation (IP): suitable
• Isotipo: IgG
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: mouse ... Akt1(11651)

Descrição

Descrição geral

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Especificidade

Recognizes Akt1/PKBα.

Imunogênio

GST-fusion protein corresponding to residues 1-149 of human Akt1

Aplicação

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Qualidade

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

Descrição-alvo

~ 60 kDa observed

forma física

20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.

Format: Purified

Protein G Chromatography

Armazenamento e estabilidade

Stable for 1 year at 2-8ºC from date of receipt.

Nota de análise

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2