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16-157

Sigma-Aldrich

Protein A Agarose/Salmon Sperm DNA, 2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

Sinônimo(s):

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.32

fonte biológica

Staphylococcus aureus

Nível de qualidade

100

Formulário

liquid

fabricante/nome comercial

Upstate®

técnica(s)

ChIP: suitable

adequação

suitable for immunoprecipitation

Condições de expedição

wet ice

Descrição geral

Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Aplicação

Suitable for use in chromatin immunoprecipitation (ChIPs) assays

Qualidade

Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)

forma física

10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

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Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications.
Yao H, Hwang JW, Moscat J, Diaz-Meco MT, Leitges M, Kishore N, Li X, Rahman I
The Journal of Biological Chemistry null
Methods for analyzing histone citrullination in chromatin structure and gene regulation.
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Mohammad Kamran et al.
Journal of immunology (Baltimore, Md. : 1950), 205(12), 3311-3318 (2020-11-15)
IL-13 plays a critical role in mediating many biological processes responsible for allergic inflammation. Mast cells express Il13 mRNA and produce IL-13 protein in response to antigenic stimulation. Enhancers are essential in promoting gene transcription and are thought to activate
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