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17-295

Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Sinônimo(s):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.41

Nível de qualidade

100

fabricante/nome comercial

EZ-ChIP

técnica(s)

immunoprecipitation (IP): suitable

Condições de expedição

wet ice

Descrição geral

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Embalagem

Kit capacity: 22 Assays

Componentes

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Qualidade

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

forma física

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H319,H412

Classificações de perigo

Aquatic Chronic 3 - Eye Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding.
Tonia R Wooldridge, Laimonis A Laimins
Virology null
Bovine Foamy Virus Transactivator (BTas) Interacts with Cellular RelB to Enhance Viral Transcription.
Wang J, Tan J, Guo H, Zhang Q, Jia R, Xu X, Geng Y, Qiao W
Journal of virology null
Chromatin immunoprecipitation for identifying transcription factor targets in keratinocytes.
Kori Ortt,Satrajit Sinha
Methods in Molecular Biology null
D Pannell et al.
The EMBO journal, 19(21), 5884-5894 (2000-11-04)
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence
Orexin signaling mediates the antidepressant-like effect of calorie restriction.
Lutter, M; Krishnan, V; Russo, SJ; Jung, S; McClung, CA; Nestler, EJ
The Journal of Neuroscience null
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