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Key Documents

1.52021

Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column

Sinônimo(s):

Chromolith® HighResolution RP-18 endcapped 50-4.6 HPLC column

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About This Item

Código UNSPSC:
41115709
NACRES:
SB.52

product name

Chromolith® HighResolution RP-18 com substituição dos grupos silanol (endcapped), L × I.D. 50 mm x 4.6 mm HPLC column

Materiais

PEEK column

Nível de qualidade

Agency

suitable for USP L1

linha de produto

Chromolith®

Características

endcapped
endcapped

embalagem

1 ea of

Extensão da rotulagem

15% Carbon loading
18% Carbon loading

Parâmetros

200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.

técnica(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

coluna C × D.I.

5 cm × 4.6 mm

matriz

highly porous
monolithic silica
monolithic silica

Grupo ativo da matriz

C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase

tamanho de poro

1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity

operating pH range

2-7.5
2-7.5

técnica de separação

reversed phase

temperatura de armazenamento

no temp limit

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Descrição geral

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping.

Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for very fast separations on any HPLC System and rapid method devlopment.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column).
[151467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: [UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns]

Nota de análise

Pressure: 15 - 50 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 135000
Capacity factor (Anthracene): 3.2 - 4.2
Tailing (USP): 0.90 - 1.35 %
Selectivity
separation factor (anthracene/progesterone): 1.90 - 2.35

Informações legais

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

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