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Merck
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Documentos Principais

1.50661

Supelco

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 50 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Sinônimo(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

Código UNSPSC:
41115709
NACRES:
SB.52

Nome do produto

SeQuant® ZIC®-cHILIC, 3µm, 100Å, 150 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC

Materiais

PEEK-lined stainless steel column

Nível de qualidade

Agency

suitable for USP L114

linha de produto

SeQuant®

Características

endcapped: no

Parâmetros

500 bar pressure
70 °C max. temp.

técnica(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

coluna C × D.I.

15 cm × 4.6 mm

Matriz

fully porous particle

Grupo ativo da matriz

ZIC-HILIC bonding phase

tamanho de partícula

3 μm

tamanho de poro

100 Å pore size

pH operacional

2-7.5

técnica de separação

hydrophilic interaction (HILIC)

temperatura de armazenamento

no temp limit

Descrição geral

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 150 mm length x 4.6 mm ID is the best choice for the separation of polar compounds using a standard HPLC Instrument.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Nota de análise

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Informações legais

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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