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Documentos Principais

14-392

Millipore

Green Fluorescent Protein

Sinônimo(s):

GFP

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About This Item

Código UNSPSC:
12352200
eCl@ss:
32160405
NACRES:
NA.32

recombinante

expressed in E. coli

Nível de qualidade

Ensaio

>70%

Formulário

liquid

peso molecular

Mw ~28 kDa

embalagem

pkg of 300 μg

fabricante/nome comercial

Upstate®

técnica(s)

western blot: suitable

nº de adesão GenBank

nº de adesão UniProt

Condições de expedição

dry ice

Descrição geral

N-terminal HIS-tagged fusion protein corresponding to full-length Green Fluorescent Protein, expressed in E. coli. Green fluorescent protein (GFP) is a cytoplasmic protein found in jellyfish Aequorea Victoria.

Ações bioquímicas/fisiológicas

Green fluorescent protein (GFP) is genetically fused to many proteins in various species to generate non-reactive chimeras which are known to preserve their original biological activity as well as the fluorescent properties of native GFP. GFP acts as a reporter for gene expression and as a fusion tag for protein localization studies in live cells. It serves as a spectroscopic and microscopic probe to understand the biological functions of various systems. GFP is highly stable and can serve as a tool for in vitro studies.

Qualidade

Routinely evaluated as a standard on protein gels and western immunoblots, for calibration of fluorometers, and microinjection into cells and tissues.

forma física

PBS containing 20% glycerol.

Armazenamento e estabilidade

1 year at -20°C

Informações legais

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Recombinant Escherichia coli (E. coli) bacteria expressing green fluorescent protein (GFP) was used as a model system to investigate the antimicrobial activities of Ag nanoparticles (NPs). A convenient in situ method of Ag NP synthesis using sodium borohydride, in the
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Autofluorescence of endogenous molecules can provide valuable insights in both basic research and clinical applications. One such technique is fluorescence lifetime imaging (FLIM) of NAD(P)H, which serves as a correlate of glycolysis and electron transport chain rates in metabolically active

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