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C2734

Sigma-Aldrich

5-Carboxytetramethylrhodamine

Sinônimo(s):

5-TAMRA

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About This Item

Fórmula empírica (Notação de Hill):
C25H22N2O5
Número CAS:
91809-66-4
Peso molecular:
430.45
Número MDL:
MFCD01073638
Código UNSPSC:
12171500
NACRES:
NA.47

Ensaio

≥95% (HPLC)

Nível de qualidade

100

forma

solid

solubilidade

water: slightly soluble

ε (coeficiente de extinção)

≥85000-97000 at 539-545 nm in methanol

fluorescência

λex ~543 nm; λem ~570 nm in methanol

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

chave InChI

YMZMTOFQCVHHFB-UHFFFAOYSA-N

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Descrição geral

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Aplicação

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Ações bioquímicas/fisiológicas

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
MKCQ1970
MKCM5246
MKCH9058
MKCG8513
MKCF9257

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Sigma-Aldrich

87918

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Azide-fluor 545

Sigma-Aldrich

760757

Azide-fluor 545

G Vámosi et al.
Biophysical journal, 71(2), 972-994 (1996-08-01)
Fluorescence steady-state and lifetime experiments have been carried out on duplex and single-stranded DNA molecules labeled at the 5' ends with 5-carboxytetramethylrhodamine (TMRh). The temperature and ionic strength of the solutions were varied over large ranges. The results reveal at
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