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Merck
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Key Documents

12-500

Sigma-Aldrich

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

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About This Item

Código UNSPSC:
41105330
eCl@ss:
32011807
NACRES:
NA.42

fonte biológica

human

Nível de qualidade

forma

liquid

fabricante/nome comercial

Upstate®

técnica(s)

western blot: suitable

Condições de expedição

dry ice

Descrição geral

EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)

Descrição de linhagem celular

HeLa

forma física

100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.

Armazenamento e estabilidade

Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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