116816

Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt

A potent, cell-permeable, and reversible metabolically-stable cAMP antagonist that inhibits cAMP-dependent protein kinase and shows preference for PKA type I.

• Sinônimo(s): Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt, Rp-8-Br-cAMPS, Na, PKA Inhibitor VI
• Fórmula empírica (Notação de Hill): C₁₀H₁₀BrN₅O₅PS · Na
• Peso molecular: 446.15
• Código UNSPSC: 41106305
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥99% (HPLC)
• forma: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic)
• solubilidade: water: 40 mg/mL
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A potent, cell-permeable, and reversible metabolically-stable cAMP antagonist that inhibits cAMP-dependent protein kinase and shows preference for PKA type I. More lipophilic than cAMP antagonist Rp-cAMPS (Cat. No. 116814).

Cell-permeable, potent, reversible, and metabolically stable antagonist of cAMP that is resistant to the action of mammalian cyclic nucleotide phosphodiesterases. Rp-8-Br-cAMPS is more lipophilic as compared to 8-Br-cAMP and Rp-cAMPS. In contrast to other common ATP-site inhibitors or to its parent compound Rp-cAMPS, Rp-8-Br-cAMPS discriminates between both isozymes of protein kinase A, preferring type I, thus providing additional selectivity. By occupying cAMP binding sites, prevents dissociation and activation of the kinase holoenzyme. λ = 264 nm (H₂O, pH 7.0); ε_max 17,000 M^-1cm^-1.

Note: 5 µmol = 2.23 mg.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
PKA 1

Product does not compete with ATP.

Reversible: yes

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Outras notas

Calaghan, S.C., et al. 1999. Pflugers Arch. 437, 780.
Gjertsen, B.T., et al. 1995. J. Biol. Chem. 270, 20599.
Yokozaki, H., et al. 1992. Cancer Res.52, 2504.
Dostmann, W.R. et al. 1990. J. Biol. Chem.265, 10484.

Informações legais

Sold under license of Patent DE 3,802,865.4 issued to BIOLOG LSI.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable