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Merck
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Key Documents

1.04141

Millipore

Singlepath® E. coli O157 Kit

suitable for immunological and lateral flow assay in Food and Beverages applications

Sinônimo(s):

Rapid E. Coli Food Test

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About This Item

Código UNSPSC:
41116129
NACRES:
NA.24

product name

Singlepath® E. coli, suitable for lateral flow assay, for Escherichia coli, input: food(s)

Agency

according to AOAC® PTM

Nível de qualidade

linha de produto

Singlepath®

prazo de validade

24 mo.

embalagem

pack of 25 strips

fabricante/nome comercial

Singlepath®

técnica(s)

lateral flow assay: suitable
microbe id | immunological: suitable

entrada

food(s)

aplicação(ões)

food and beverages
pathogen testing

temperatura de armazenamento

2-8°C

adequação

Escherichia coli (O157)
enterovirulent E. coli (EEC)

Descrição geral

Singlepath® E. coli is a rapid diagnostic test, designed to detect E. coli bacteria in various food and environmental samples. The Singlepath® E. coli is an immunochromatographic rapid test based on gold-labeled antibodies to detect the presence of a specific antigen, in this case, the E. coli bacteria. This lateral flow test is based on the immune flow principle and is a convenient and simple-to-use, one-step device. The test includes a nitrocellulose membrane strip that is coated with antibodies specific to E. coli antigens. When the sample is applied to the test strip, any E. coli antigens present will bind to the antibodies on the strip, resulting in the development of a visible test line. The test takes approximately 20 minutes to complete, and the results are interpreted based on the presence or absence of the test line. The detection limit can be regarded as less than one colony-forming unit of E. coli O157 (including H7) in a 25 g food sample, depending on the serogroup. No additional equipment expenditure is needed.

Aplicação

SINGLEPATH® E. coli O157 is used for pathogen detection in industrial microbiology. This kit is designed for testing food and environmental samples. The test is intended to be used in food-analyzing laboratories for the presumptive qualitative detection of E. coli O157 (including H7) from a variety of foods. The test has been validated and approved for use in raw ground beef and pasteurized whole milk.

Características e benefícios

  • Rapid and reliable method
  • High sensitivity and specificity compared to conventional culture methods
  • Can be performed without the need for specialized equipment or personnel.

Ligação

Replaces: 1.04141.0002

Nota de análise

Performance test
- Escherichia coli O157:H7 ATCC 35150:
passes test
- E. coli O157:H7 HUSCL8: passes test
- E. coli O55:H7 TB182A: passes test

Informações legais

AOAC is a registered trademark of AOAC International
SINGLEPATH is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids


Certificados de análise (COA)

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