09-432

Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody

Upstate^®, from rabbit

• Sinônimo(s): HIRS-1, Insulin Receptor Substrate 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• fabricante/nome comercial: Upstate^®
• técnica(s): western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NM_005544
• nº de adesão UniProt: P35568
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: phosphorylation (pTyr608 )
• Informações sobre genes: human ... IRS1(3667)

Descrição

Descrição geral

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Especificidade

Predicted to cross react with mouse and rat based on 100% sequence homology.

Recognizes phospho-IRS1 (Tyr608) mouse/ (Tyr612) human

Imunogênio

peptide corresponding to amino acids encompassing and including phosphorylated Tyr608 in mouse and Tyr612 in human of IRS1

Aplicação

Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody detects level of phospho-IRS1 (Tyr608) mouse/ (Tyr612) human & has been published & validated for use in WB.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Qualidade

Evaluated by Western Blotting in Insulin treated HEK293 cell lysate.

Western Blotting Analysis: 1 mg/mL of this antibody detected phospho-IRS1 (Tyr608) mouse/ (Tyr612) in 10 µg of lysate from Insulin treated HEK293 cells.cells

Descrição-alvo

~160 kDa observed. 131.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Affinity purified

Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol.

Armazenamento e estabilidade

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable