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Documentos Principais

09-019

Sigma-Aldrich

Anti-REST Antibody

from rabbit, purified by affinity chromatography

Sinônimo(s):

Neural-restrictive silencer factor, RE1-silencing transcription factor, X2 box repressor, neuron restrictive silencer factor, repressor binding to the X2 box

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse, rat, human

técnica(s)

western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... REST(5978)
mouse ... Rest(19712)
rat ... Rest(83618)

Descrição geral

REST (RE1-silencing transcription factor) also known as NRSF (neuron-restrictive silencer factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of REST to the NRSE represses neuronal gene transcription in non-neuronal cells. Although REST is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. REST contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. REST associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for REST outside the nervous system. Down-regulation of REST, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. REST is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

Especificidade

Recognizes REST

Imunogênio

Epitope: C-terminus
GST fusion protein corresponding to residues 801-1097 of human REST.

Aplicação

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Signaling Neuroscience
This Anti-REST Antibody is validated for use in WB for the detection of REST.

Qualidade

Evaluated by western blot with HeLa nuclear extract.

Western Blot Analysis:
0.5-1 μg/mL of this antibody detected REST in nuclear extracts from HeLa, Raji, and Jurkat cells.

Descrição-alvo

120 kDa

forma física

Affinity purified rabbit serum in PBS with 0.07% sodium azide, and 30% glycerol.
Antigen Affinity Purified

Armazenamento e estabilidade

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Nota de análise

Control
HeLa nuclear extract

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Loss of the repressor REST in uterine fibroids promotes aberrant G protein-coupled receptor 10 expression and activates mammalian target of rapamycin pathway.
Varghese, BV; Koohestani, F; McWilliams, M; Colvin, A; Gunewardena, S; Kinsey, WH; Nowak et al.
Proceedings of the National Academy of Sciences of the USA null
Pei-Ching Chang et al.
PloS one, 9(2), e88556-e88556 (2014-02-20)
Prostate cancer (PCa) cells undergoing neuroendocrine differentiation (NED) are clinically relevant to the development of relapsed castration-resistant PCa. Increasing evidences show that autophagy involves in the development of neuroendocrine (NE) tumors, including PCa. To clarify the effect of autophagy on

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