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Merck
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Documentos Principais

07-488

Sigma-Aldrich

Anti-PKD2 Antibody

Upstate®, from rabbit

Sinônimo(s):

Serine/threonine-protein kinase D2, nPKC-D2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

human

fabricante/nome comercial

Upstate®

técnica(s)

western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PKD2(353503)

Especificidade

Recognizes PKD2.

Imunogênio

KLH-conjugated, synthetic peptide corresponding to amino acids 850-865 (GLPTDRDLGGACPPQD) of human protein kinase D2 (PKD2).

Aplicação

Anti-PKD2 Antibody detects level of PKD2 & has been published & validated for use in WB.
Immunoblot Analysis: A 1:500-1:1,000 dilution of this antibody detected PKD2 in RIPA lysates from A431 cells that had been stimulated with PMA.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Qualidade

Routinely evaluated by immunoblot in RIPA lysates from HEK293m cells that had been stimulated with 50 ng/mL of PDBu for 10 minutes or PMA stimulated A431 cells.

Descrição-alvo

105 kDa

forma física

ImmunoAffinity Purified
Immunoaffinity purified rabbit IgG in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide as a preservative.

Armazenamento e estabilidade

Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análise

Control
PMA stimulated A431 cells

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Ying Wang et al.
The Journal of biological chemistry, 294(43), 15759-15767 (2019-09-08)
Vascular endothelial growth factor A (VEGF) signals primarily through its cognate receptor VEGF receptor-2 (VEGFR-2) to control vasculogenesis and angiogenesis, key physiological processes in cardiovascular disease and cancer. In human umbilical vein endothelial cells (HUVECs), knockdown of protein kinase D-1
Protein kinase d as a potential chemotherapeutic target for colorectal cancer.
Wei, N; Chu, E; Wipf, P; Schmitz, JC
Molecular Cancer Therapeutics null
Nisha Durand et al.
Oncotarget, 9(91), 36358-36370 (2018-12-18)
Phosphatidylinositol-4-phosphate 5-kinase type-1C (PIP5K1C) is a lipid kinase that regulates focal adhesion dynamics and cell attachment through site-specific formation of phosphatidylinositol-4,5-bisphosphate (PI4,5P2). By comparing normal breast tissue to carcinoma in situ and invasive ductal carcinoma subtypes, we here show that
Sabine Sturany et al.
The Journal of biological chemistry, 277(33), 29431-29436 (2002-06-12)
Recently, we cloned a novel serine/threonine kinase termed protein kinase D2 (PKD2). PKD2 can be activated by phorbol esters both in vivo and in vitro but also by gastrin via the cholecystokinin/CCK(B) receptor in human gastric cancer cells stably transfected
Xueyu Fan et al.
Cancer medicine, 7(6), 2541-2554 (2018-04-17)
Cancer cells undergo comprehensive metabolic reprogramming to meet the increased requirements of energy and building blocks for proliferation. Lipin-1, a phosphatidic acid phosphatase converting phosphatidic acid (PA) to diacylglycerol (DAG), is upregulated in lung adenocarcinoma (LUAD) cell lines and tumor

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