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Key Documents

07-452

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Sinônimo(s):

H3K27me2, Histone H3 (di methyl K27)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

100

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

human

fabricante/nome comercial

Upstate®

técnica(s)

ChIP: suitable (ChIP-seq)
cell based assay: suitable
dot blot: suitable
multiplexing: suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

NM_003493

nº de adesão UniProt

Q16695

Condições de expedição

wet ice

modificação pós-traducional do alvo

dimethylation (Lys27)

Informações sobre genes

human ... H3C1(8350)

Descrição geral

Anti-dimethyl-Histone H3 (Lys27)

Especificidade

Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).

Imunogênio

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3

Aplicação

Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).

Qualidade

Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.

Descrição-alvo

17 kDa

forma física

Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified

Armazenamento e estabilidade

Stable for 2 years at 2-8°C from date of shipment.

Nota de análise

Control
HeLa whole cell lysate, U2OS cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response.
Wen, H; Dou, Y; Hogaboam, CM; Kunkel, SL
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Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5.
Xu, CR; Schaffer, L; Head, SR; Feeney, AJ
Proceedings of the National Academy of Sciences of the USA null
Mathieu Lupien et al.
Cell, 132(6), 958-970 (2008-03-25)
Complex organisms require tissue-specific transcriptional programs, yet little is known about how these are established. The transcription factor FoxA1 is thought to contribute to gene regulation through its ability to act as a pioneer factor binding to nucleosomal DNA. Through
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