06-991

Anti-PKCε Antibody

Upstate^®, from rabbit

• Sinônimo(s): protein kinase C, epsilon
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: rat, human, mouse
• fabricante/nome comercial: Upstate^®
• técnica(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NM_005400
• nº de adesão UniProt: Q02156
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PRKCE(5581)

Descrição

Descrição geral

Members of the protein kinase C family fall into three categories: conventional (PKC alpha, beta I, beta II, gamma), novel (delta, epsilon, eta, mu, theta), and atypical (lambda, zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.

Especificidade

Predicted to cross-react with rabbit.

Recognizes PKCε, Mr 95 kDa.

Imunogênio

KLH-conjugated, synthetic peptide corresponding to the C-terminal variable (V5) region, amino acids 726-737 (KGFSYFGEDLMP), of mouse Protein Kinase C Epsilon (PKCε). The immunizing sequence is identical in human, mouse, rat and rabbit.

Aplicação

Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.

Immunoprecipitation:
An independent lab has reported this antibody immunoprecipitates PKCε.

Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Qualidade

Routinely evaluated by Western Blot on Jurkat cell lysate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.

Descrição-alvo

Approx. 95

forma física

Format: Purified

Protein A purified

Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Store at -20°C.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Nota de análise

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1