05-745R
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal
clone 15-10C-E4, Upstate®, from rabbit
Sinônimo(s):
H3K4me3, Histone H3 (tri methyl K4)
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
15-10C-E4, monoclonal
reatividade de espécies
human
fabricante/nome comercial
Upstate®
técnica(s)
ChIP: suitable (ChIP-seq)
ELISA: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
Isotipo
IgG
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
trimethylation (Lys4)
Informações sobre genes
human ... H3F3B(3021)
Descrição geral
Recombinant version of Cat. # 05-745, clone MC315
Especificidade
Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See certificate of analysis for further details.
Imunogênio
BSA-conjugated, synthetic peptide containing the sequence …RTme3KQT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3.
Aplicação
ChIP-seq Analysis:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 3 µL of Anti-trimethyl-Histone H3 (Lys4) antibody (cat# 05-745R), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 05-745R and 07-473 datasets showed 99% overlap with peaks identified in the ENCODE H3K4me3 BROAD Histone track for HeLa S3.
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 3 µL of Anti-trimethyl-Histone H3 (Lys4) antibody (cat# 05-745R), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 05-745R and 07-473 datasets showed 99% overlap with peaks identified in the ENCODE H3K4me3 BROAD Histone track for HeLa S3.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Recombinant (rabbit monoclonal antibody) proven in WB, ChIP, ChIP-seq, ELISA, PIA, Mplex, DB to detect trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4).
Qualidade
Routinely evaluated by immunoblot on HeLa nuclear extract
Descrição-alvo
17 kDa
Ligação
Replaces: 05-745
forma física
Format: Purified
Protein A purified, recombinant antibody in 60 mM Tris, 90 mM NaCl, 0.03% NaN3, 60 mM Glycine, pH 7.5, 40% Glycerol.
Armazenamento e estabilidade
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Lakshmi Bugga et al.
PloS one, 8(3), e59496-e59496 (2013-03-28)
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Coordinate H3K9 and DNA methylation silencing of ZNFs in toxicant-induced malignant transformation.
Severson, PL; Tokar, EJ; Vrba, L; Waalkes, MP; Futscher, BW
Epigenetics null
Simon M G Braun et al.
Nature communications, 8(1), 560-560 (2017-09-17)
Understanding the causal link between epigenetic marks and gene regulation remains a central question in chromatin biology. To edit the epigenome we developed the FIRE-Cas9 system for rapid and reversible recruitment of endogenous chromatin regulators to specific genomic loci. We
A map of the cis-regulatory sequences in the mouse genome.
Shen, Yin, et al.
Nature, 488, 116-120 (2012)
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EBioMedicine, 62, 103108-103108 (2020-11-14)
Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide, with unmet need for the pharmacological therapy. The functions of ATXN7L3 in HCC progression are not known. RNA sequence, quantitative real-time PCR, and western blot were performed to detect
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