05-637-AF647

Anti-Bmi-1 Antibody, clone F6, Alexa Fluor^™ 647

clone F6, from mouse, ALEXA FLUOR^™ 647

• Sinônimo(s): Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, Bmi-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: F6, monoclonal
• application: ICC
• reatividade de espécies: human, monkey
• técnica(s): immunocytochemistry: suitable

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: ALEXA FLUOR^™ 647
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: F6, monoclonal
• reatividade de espécies: human, monkey
• reatividade da espécie (prevista por homologia): rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnica(s): immunocytochemistry: suitable
• Isotipo: IgG1
• nº de adesão NCBI: NP_031578.2
• nº de adesão UniProt: P25916
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... BMI1(648)

Descrição

Descrição geral

Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.

Imunogênio

Epitope: a.a. 1-202

Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Aplicação

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HepG2 cells.
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor^™ 647 is validated for use in Immunocytochemistry for the detection of Bmi-1.

Qualidade

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HeLa cells.

Descrição-alvo

40-44 kDa observed

forma física

Protein G Purified

Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable