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Documentos Principais

05-1245

Sigma-Aldrich

Anti-O-GlcNAc Antibody, clone 9D1.E4(10)

clone 9D1.E4(10), from mouse

Sinônimo(s):

O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked GlcNAc transferase, O-linked N-acetylglucosamine (GlcNAc) transferase

(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, O-linked N-acetylglucosam

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

9D1.E4(10), monoclonal

reatividade de espécies

human

técnica(s)

western blot: suitable

Isotipo

IgG1

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

glycosylation

Informações sobre genes

human ... OGT(8473)

Descrição geral

O-linked N-acetylglucosamine (O-GlcNAc), is a member of the O-GlcNAc transferase family. O-GlcNAc is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine/threonine hydroxyl groups. It plays an important role in nutrient sensing, gene expression, and protein degradation. O-GlcNAc is widely expressed in the pancreas and is present in smaller amounts in skeletal muscle, heart, brain and placenta.

Especificidade

This antibody recognizes O-GlcNAc.

Imunogênio

SANM-conjugated linear peptide corresponding to human O-GlcNAc.

Aplicação

Detect O-GlcNAc using this Anti-O-GlcNAc Antibody, clone 9D1.E4(10) validated for use in WB.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Qualidade

Evaluated by Western Blot in O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected O-GlcNAc on 10 µg of
O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.

Descrição-alvo

Various

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Maintain refrigerated at 2-8°C for up to 12 months.

Nota de análise

Control
HEK293 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Glucose deprivation-induced increase in protein O-GlcNAcylation in cardiomyocytes is calcium-dependent.
Zou, L; Zhu-Mauldin, X; Marchase, RB; Paterson, AJ; Liu, J; Yang, Q; Chatham, JC
The Journal of Biological Chemistry null
Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress.
Reeves, RA; Lee, A; Henry, R; Zachara, NE
Analytical biochemistry null
Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc.
Teo, Chin Fen, et al.
Nature chemical biology, 6, 338-343 (2010)
Yuko Tashima et al.
The Journal of biological chemistry, 289(16), 11132-11142 (2014-02-28)
The transfer of N-acetylglucosamine (GlcNAc) to Ser or Thr in cytoplasmic and nuclear proteins is a well known post-translational modification that is catalyzed by the O-GlcNAc transferase OGT. A more recently identified O-GlcNAc transferase, EOGT, functions in the secretory pathway

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