04-820
Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
culture supernatant, clone 60C, from rabbit
Sinônimo(s):
Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, estrogen receptor 1, estrogen receptor alpha, oestrogen receptor, steroid hormone receptor
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
culture supernatant
tipo de produto de anticorpo
primary antibodies
clone
60C, monoclonal
reatividade de espécies
rat, pig, mouse, human, bovine
embalagem
antibody small pack of 25 μL
técnica(s)
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
bovine ... Esr1(407238)
human ... ESR1(2099)
mouse ... Esr1(13982)
pig ... Esr1(397435)
rat ... Esr1(24890)
Descrição geral
The Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.
Especificidade
Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology.
Recognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β.
Imunogênio
Epitope: N-term
KLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine.
Aplicação
Anti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP.
Immunoprecipitation:
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualidade
Routinely evaluated by western blot analysis.
Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Descrição-alvo
66 kDa
Ligação
Replaces: 05-820
forma física
Cultured supernantant with 0.05% sodium azide.
Unpurified
Armazenamento e estabilidade
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Florent Morfoisse et al.
Arteriosclerosis, thrombosis, and vascular biology, 38(6), 1346-1357 (2018-04-14)
Estrogens exert beneficial effect on the blood vascular system. However, their role on the lymphatic system has been poorly investigated. We studied the protective effect of the 17β estradiol-the most potent endogenous estrogen-in lymphedema-a lymphatic dysfunction, which results in a
Coralie Poulard et al.
EMBO molecular medicine, 4(11), 1200-1213 (2012-10-16)
Oestrogen receptors can mediate rapid activation of cytoplasmic signalling cascades by recruiting Src and PI3K. However, the involvement of this pathway in breast cancer remains poorly defined. We have previously shown that methylation of ERα is required for the formation
Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.
Bolt, MJ; Stossi, F; Newberg, JY; Orjalo, A; Johansson, HE; Mancini, MA
Nucleic Acids Research null
Victoria L Nutsch et al.
Hormones and behavior, 96, 4-12 (2017-09-09)
Testosterone is the main circulating steroid hormone in males, and acts to facilitate sexual behavior via both reduction to dihydrotestosterone (DHT) and aromatization to estradiol. The mPOA is a key site involved in mediating actions of androgens and estrogens in
Defining estrogenic mechanisms of bisphenol A analogs through high throughput microscopy-based contextual assays.
Stossi, F; Bolt, MJ; Ashcroft, FJ; Lamerdin, JE; Melnick, JS; Powell, RT; Dandekar et al.
Chemistry & Biology null
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica