04-1564

Anti-Estrogen Receptor α Antibody, clone F3-A

ascites fluid, clone F3-A, from mouse

• Sinônimo(s): Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, estrogen receptor 1, estrogen receptor alpha, estrogen receptor alpha delta 4*,5,6,7*/633 isoform, estrogen receptor alpha delta 6,7 isoform
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: F3-A, monoclonal
• reatividade de espécies: rat, human, mouse
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_000116
• nº de adesão UniProt: P03372
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ESR1(2099)

Descrição

Descrição geral

Estrogen Receptor α (ERα) is a 65 kDa nuclear hormone receptor. This steroid hormone receptor is involved in the regulation of eukaryotic gene expression and affects cellular proliferation and differentiation in target tissues. ERα is a ligand-activated transcription factor and upon being bound by a ligand such as estrogen, a conformational change occurs that allows dimerization and binding to an estrogen response element. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Two ERs are known, ERα, classically known as ER, and ERβ. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.

Especificidade

This antibody recognizes Estrogen Receptor α.

Imunogênio

Epitope: Unknown

Linear peptide corresponding to human Estrogen Receptor α.

Aplicação

Anti-Estrogen Receptor α Antibody, clone F3-A is a mouse monoclonal antibody for detection of Estrogen Receptor α also known as Estradiol receptor & Nuclear receptor subfamily 3 group A member 1 has been validated in WB, ICC.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Estrogen Receptor α in A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualidade

Evaluated by Western Blot in mouse ovary tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Estrogen Receptor α on 10 µg of mouse ovary tissue lysate.

Descrição-alvo

~ 67 kDa

forma física

Unpurified

Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Mouse ovary tissue lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable