04-797
Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39
clone Aw39, Upstate®, from rabbit
Sinônimo(s):
Mitogen-activated protein kinase, Extracellular signal-regulated kinase, protein tyrosine kinase ERK2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
culture supernatant
tipo de produto de anticorpo
primary antibodies
clone
Aw39, monoclonal
reatividade de espécies
human, rat
fabricante/nome comercial
Upstate®
técnica(s)
multiplexing: suitable
western blot: suitable
Isotipo
IgG
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
phosphorylation (pThr185/pTyr187)
Informações sobre genes
human ... MAPK1(5594)
rat ... Mapk1(116590)
Descrição geral
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).
Especificidade
Predicted to cross-react with many species based on sequence homology.
Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).
Imunogênio
KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.
Aplicação
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex microspheres. (Figure B).
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex microspheres. (Figure B).
Qualidade
Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells
Descrição-alvo
MAPK1 (~44 kDa) and MAPK2 (~42 kDa).
Ligação
Replaces: 05-797
forma física
Cultured supernantant in 0.05% sodium azide
Format: Purified
Armazenamento e estabilidade
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análise
Control
RIPA cell lysate from NGF stimulated PC-12 cells
RIPA cell lysate from NGF stimulated PC-12 cells
Outras notas
Concentration: Variable
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Sandra L Krueckl et al.
Cancer research, 64(23), 8620-8629 (2004-12-03)
Apoptosis and inhibition of mitosis are primary mechanisms mediating androgen ablation therapy-induced regression of prostate cancer (PCa). However, PCa readily becomes androgen independent, leading to fatal disease. Up-regulated growth and survival signaling is implicated in development of resistance to androgen
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