04-1664

Anti-Histidine Tag Antibody, clone RM146

clone RM146, from rabbit

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: RM146, monoclonal
• reatividade da espécie (prevista por homologia): all
• técnica(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HRG(3273)

Descrição

Especificidade

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Imunogênio

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Aplicação

Immunoprecipitation Analysis (IP): 0.5-2 μg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.

Qualidade

Evaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.

Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.

Descrição-alvo

~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein A purified

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash