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Key Documents

700147P

Avanti

PhotoClick Cholesterol

Hex-5′-ynyl 3β-hydroxy-6-diazirinyl-5α-cholan-24-oate, powder

Sinônimo(s):

hex-5-yn-1-yl 4-((3S,5S)-3-hydroxy-10, 13-dimethyl-1,2,3,4,5,7,8,9,10,11,12,13,14,15,16,17-hexadecahydrospiro[cyclopenta[a]phenanthrene-6,3′-diazirin]-17-yl)pentanoate

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About This Item

Fórmula empírica (Notação de Hill):
C30H46N2O3
Número CAS:
1485490-47-8
Peso molecular:
482.70
Código UNSPSC:
12352211
NACRES:
NA.25

Ensaio

>99% (TLC)

forma

powder

embalagem

pkg of 1 × 100 μg (with stopper and crimp cap (700147P-100ug))
pkg of 1 × 250 μg (with stopper and crimp cap (700147P-250ug))

fabricante/nome comercial

Avanti Research™ - A Croda Brand 700147P

tipo de lipídio

click reagents

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição geral

PhotoClick Cholesterol is a clickable, photoreactive sterol probe for use in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. In addition to the UV-activatable cross-linking diazirine group that reacts with proteins, the sterol contains an alkyne group for incorporation of reporter and affinity tags through click-chemistry.
PhotoClick Cholesterol is a synthetic analog of cholesterol and is photoreactive. It comprises the alkyne group.

Aplicação

PhotoClick Cholesterol has been used:
  • in lysosome-associated membrane protein 1 (LAMP1) crosslinking studies
  • as an analog of cholesterol in binding and competition assay of sterol binding proteins from yeast
  • as a conjugate with the ectodomain of lysosomal integral membrane protein-2 (LIMP-2)

Ações bioquímicas/fisiológicas

PhotoClick Cholesterol serves as a probe for tracking cholesterol-binding proteins. The copper-catalyzed azide-alkyne cycloaddition is the basis for its probe functionality. The alkyne group is essential for the click chemistry property. Its binding to partners is mediated by photocrosslinking.

Embalagem

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-100ug)
2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-250ug)

Informações legais

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Código de classe de armazenamento

11 - Combustible Solids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Neha Chauhan et al.
Yeast (Chichester, England), 37(1), 15-25 (2019-11-24)
Ergosterol is a prominent component of the yeast plasma membrane and essential for yeast cell viability. It is synthesized in the endoplasmic reticulum and transported to the plasma membrane by nonvesicular mechanisms requiring carrier proteins. Oxysterol-binding protein homologues and yeast
Jonathan J Hulce et al.
Nature methods, 10(3), 259-264 (2013-02-12)
Cholesterol is an essential structural component of cellular membranes and serves as a precursor for several classes of signaling molecules. Cholesterol exerts its effects and is, itself, regulated in large part by engagement in specific interactions with proteins. The full
Saskia Heybrock et al.
Nature communications, 10(1), 3521-3521 (2019-08-08)
The intracellular transport of cholesterol is subject to tight regulation. The structure of the lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) reveals a large cavity that traverses the molecule and resembles the cavity in SR-B1 that

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