906603
DLAM-Iδ1Tγ-13CH3 Methyl Labeling Kit
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About This Item
Produtos recomendados
técnica(s)
bio NMR: suitable
Nível de qualidade
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Descrição geral
DLAM-Iδ1Tg-13CH3 kit has 13CH3isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.
Aplicação
DLAM-Iδ1Tg-13CH3 is an optimized kit to produce proteins with 13CH3 isotopomer on the methyl groups of isoleucine and threonine. This kit has been tested with protein isotopic labeling in E. coli. It is used in methyl-TROSY NMR for the investigation of the active site of the proteasome and also in the study of catalytic mechanism.
For detection of long-range nOes or for the NMR study of large proteins
Embalagem
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
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Journal of the American Chemical Society, 135(25), 9259-9262 (2013-06-27)
The 20S proteasome core particle is a molecular machine that plays a central role in the regulation of cellular function through proteolysis, and it has emerged as a valuable drug target for certain classes of cancers. Central to the development
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
Artigos
Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.
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