MAK118
Invertase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonyme(s) :
β-fructofuranosidase Assay Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric or fluorometric tests
Application(s)
cosmetics
environmental
food and beverages
Méthode de détection
colorimetric
fluorometric
Température de stockage
−20°C
Description générale
Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.
Application
Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.
Principe
In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.
Code de la classe de stockage
10 - Combustible liquids
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Les clients ont également consulté
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Frontiers in Plant Science, 9 (2018)
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Journal of experimental botany, 67(11), 3303-3312 (2016-04-17)
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these
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Frontiers in plant science, 9, 598-598 (2018-05-19)
In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play
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