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SCA20

Supelco

Sucrose Assay Kit

sufficient for 20 assays

Synonyme(s) :

Sucrose Test Kit

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About This Item

Numéro CE :
200-334-9
Code UNSPSC :
12164500
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 20 assays

Niveau de qualité

100

Classe(s) chimique(s) de l'analyte

sugars (sucrose)

Technique(s)

photometry: suitable

Application(s)

food and beverages
general analytical

Température de stockage

2-8°C

Catégories apparentées

Description générale

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Composants de kit seuls

Réf. du produit
Description
  • glucose assay reagent 50 mL
  • sucrose assay reagent 1 mL/vial
  • sucrose standard 500 mg

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Bergmeyer, H.U. and Bernt, E.
Methods of Enzymatic Analysis, 1176-1176 (1974)
Ye Ji Lee et al.
Frontiers in plant science, 12, 644455-644455 (2021-07-20)
Nitrogen is one of the most important macro-nutrients for plant growth and crop productivity. The amount of synthetic nitrogen fertilizers supplied to crops has dramatically increased, leading to a notable rise in crop yields. However, excessive nitrogen use has an
Raja S Payyavula et al.
BMC plant biology, 12, 39-39 (2012-03-21)
Plant secondary metabolites, including phenylpropanoids and carotenoids, are stress inducible, have important roles in potato physiology and influence the nutritional value of potatoes. The type and magnitude of environmental effects on tuber phytonutrients is unclear, especially under modern agricultural management
Ching M Wai et al.
The Plant journal : for cell and molecular biology, 92(1), 19-30 (2017-07-04)
The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here
Quantitative 1H NMR with external standards: use in preparation of calibration solutions for algal toxins and other natural products.
Burton IW, Quilliam MA, Walter JA.
Analytical Chemistry, 77(10), 3123-3131 (2005)
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Protocoles

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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