Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

I3911

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, lyophilized powder, for the inhibition of serine, cysteine, aspartic and metalloproteases, for general use, lyophilized powder

Synonyme(s) :

protease inhibitor

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

product name

Protease Inhibitor Cocktail, Animal Component Free, for general use, lyophilized powder

Niveau de qualité

200

Forme

lyophilized powder

Solubilité

water: soluble

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Description générale

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.

Spécificité

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples, peritoneal residential macrophage cells and in embryo culture medium.

Composants

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Quantité

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Forme physique

Lyophilized powder that is water soluble.
The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Eye Dam. 1 - Skin Corr. 1A

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
Lopucki M, et al.
Molecular Medicine Reports, 17(6), 8253-8259 (2018)
Functional evaluation of multiple human transgenes to overcome immunological and coagulation barriers associated with pig-to-human xenotransplantation.
Bongoni, A.K. et al.
Thesis, 108-108 (2013)
Marija Holcar et al.
Scientific reports, 10(1), 21346-21346 (2020-12-09)
Human plasma is a complex fluid, increasingly used for extracellular vesicle (EV) biomarker studies. Our aim was to find a simple EV-enrichment method for reliable quantification of EVs in plasma to be used as biomarker of disease. Plasma of ten
Early protein profile of human embryonic secretome.
Foresta C, et al.
Frontiers in Bioscience, 21, 620-634 (2016)
Carlo Foresta et al.
Frontiers in bioscience (Landmark edition), 21, 620-634 (2015-12-29)
Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique