539131
Protease Inhibitor Cocktail Set I
A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.
Synonyme(s) :
Protease Inhibitor Mix, Protease inhibitor
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Forme
lyophilized
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
desiccated (hygroscopic)
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.
Spécificité
Inhibits a broad range of proteases and esterases.
Application
Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
- cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
- RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
- sucrose solution to homogenize mice cortices for myelin isolation
Actions biochimiques/physiologiques
Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.
Caractéristiques et avantages
- Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
- Ready for reconstitution
Avertissement
Toxicity: Harmful (C)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.
Autres remarques
The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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