XTG360-RO
Roche
X-tremeGENE™ 360 Transfection Reagent
Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
Synonyme(s) :
transfection reagent
About This Item
Produits recommandés
Qualité
for molecular biology
Forme
liquid (aqueous solution)
Conditionnement
pkg of 0.4 mL (08724105001)
pkg of 1.0 mL (08724121001)
pkg of 5 × 1.0 mL (08724156001)
Fabricant/nom de marque
Roche
Technique(s)
transfection: suitable
Température de stockage
−20°C
Description générale
Application
- Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
- Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
- Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
- Suitable for transient and stable transfection.
- Functions well in the presence or absence of serum.
Caractéristiques et avantages
- Peak performance in a broad range of cell types
- Suitable for multiple applications
- Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components
Qualité
Forme physique
Autres remarques
Informations légales
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
61.7 °F
Point d'éclair (°C)
16.5 °C
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Articles
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
Protocoles
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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