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  • Metabolite profile, antioxidant capacity, and inhibition of digestive enzymes in infusions of peppermint (Mentha piperita) grown under drought stress.

Metabolite profile, antioxidant capacity, and inhibition of digestive enzymes in infusions of peppermint (Mentha piperita) grown under drought stress.

Journal of agricultural and food chemistry (2014-12-03)
Marely G Figueroa-Pérez, Nuria Elizabeth Rocha-Guzmán, Iza F Pérez-Ramírez, Edmundo Mercado-Silva, Rosalía Reynoso-Camacho
ZUSAMMENFASSUNG

Peppermint (Mentha piperita) infusions represent an important source of antioxidants, which can be enhanced by inducing abiotic stress in plants. The aim of this study was to evaluate the effect of drought stress on peppermint cultivation as well as the metabolite profile, antioxidant capacity, and inhibition of digestive enzymes of resulting infusions. At 45 days after planting, irrigation was suppressed until 85 (control), 65, 35, 24, and 12% soil moisture (SM) was reached. The results showed that 35, 24, and 12% SM decreased fresh (20%) and dry (5%) weight. The 35 and 24% SM treatments significantly increased total phenolic and flavonoid contents as well as antioxidant capacity. Coumaric acid, quercetin, luteolin, and naringenin were detected only in some drought treatments; however, in these infusions, fewer amino acids and unsaturated fatty acids were identified. The 24 and 12% SM treatments slightly improved inhibition of pancreatic lipase and α-amylase activity. Therefore, induction of moderate water stress in peppermint is recommended to enhance its biological properties.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Sigma-Aldrich
Buttersäure-4-Nitrophenylester, ≥98%
Sigma-Aldrich
Luteolin, ≥98% (TLC), powder
Sigma-Aldrich
(±)-Naringenin, ≥95%
Sigma-Aldrich
Hesperidin, ≥80% (HPLC)
Sigma-Aldrich
Vanillin, ReagentPlus®, 99%
Sigma-Aldrich
Vanillin, ≥97%, FCC, FG
Sigma-Aldrich
Vanillin, natural, ≥97%, FCC, FG
USP
Quercetin, United States Pharmacopeia (USP) Reference Standard
USP
Vanillin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Naringenin, natural (US), 98%
Supelco
Vanillin Schmelzpunktstandard, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Quercetin, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Natriumnitroprussid, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Vanillin, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Kalibriersubstanz von Mettler Toledo, ME 51143093, Vanillin, traceable to primary standards (LGC)
Sigma-Aldrich
Vanillin, tested according to Ph. Eur.
Supelco
(±)-Naringenin, analytical standard
Supelco
Luteolin, analytical standard
Supelco
Hesperidin, analytical standard
Vanillin, European Pharmacopoeia (EP) Reference Standard
Supelco
Vanillin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Luteolin, primary reference standard
Hesperidin, primary reference standard
Eriocitrin, primary reference standard
Hesperidin, European Pharmacopoeia (EP) Reference Standard