SHPHLIBR

MISSION^® LentiPlex^® Complete Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

• UNSPSC-Code: 41105904
• NACRES: NA.51

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: MISSION^®
• Konzentration: ≥5x10⁸ VP/ml (via p24 assay)
• Methode(n): capture ELISA: 5 x 10⁸ TU/mL using p24
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Complete Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 125,000 shRNA constructs from the TRC collection targeting 20,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 10⁸ TU/ml via p24 assay and is pre-divided into sixteen subpools of approximately 6,000-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.

Sonstige Hinweise

The MISSION LentiPlex Human Pooled shRNA Libraries consist of Catalog Numbers SHPH01, SHPH15, SHPH2, and SHPHLIBR. Please refer to Table 1 for approximate number of shRNA clone coverage, targeted genes, and subpools for each library.

Amplification and sequencing primers are also provided for downstream hit identification.

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

• Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
• Comprehensive, reproducible results from pooled shRNA screens
• Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Rechtliche Hinweise

Lentiviral License Agreement

LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable